TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

  • 来源:生物谷
  • 时间: 2020/10/21
  • 浏览人数: 593

     TBS-T or PBS-T, does it matter which buffer is used for antibody incubations? 

    Oftentimes TBS-T is a buffer of choice applied in many Western blot protocols for all antibody incubations. 

    However, as you can see in this example, changing to PBS-T has also changed a pattern of detected bands. Analysed samples (Arabidopsis thaliana), protein load/well, primary, secondary antibodies and detection reagent were kept the same. 

    Antibody incubation buffer has been changed from TBS-T (left pannel) to PBS-T(right panel).
     


     

      Western blot with anti-catalase antibodies


    Western blot protocol with all details for this experiment can be found here