DSIL-LHRH-A1细胞

ATCC Number： HB-9094™
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
细胞形态： 淋巴样
免疫类型： IgG1
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: USASK/DSIL-LHRH-A1
Depositors: University of Saskatchewan
Isotype: IgG1
DSIL-LHRH-A1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)
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Tumorigenic: Yes
Comments:DSIL-LHRH-A1细胞Animals were immunized with LHRH conjugated to bovine serum albumin. 

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
A feeder layer is required to recover the cells from a frozen ampule. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine and 50 mg/l gentamicin, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Preservation: Culture medium, 95%; DMSO, 5%
References: 58144: DSIL-LHRH-A1细胞Silversides DW, et al. Monoclonal antibodies against LHRH: development and immunoactivity in vivo and in vitro. J. Reprod. Immunol. 7: 171-184, 1985. PubMed: 3884802