Disease:

Non-Small cell lung cancer; squamous cells 

Origin:

 Established from a donor who was a smoker (50 packs/year)

Species:

 Homo sapiens 

Tissue:

Lung 

Morphology:

Epithelial 

Properties:

 Adherent 

Patient:

 Male, Caucasian, 54 years of age 

Medium:

 RPMI-1640 Medium + 10% FBS 

Subculture:

 A subcultivation ratio of 1:2 to 1:7 is recommended.5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

DNA Profile (STR):
 Amelogenin: X,Y; CSF1PO: 12; D13S317: 10; D16S539: 10,12; D5S818: 11; D7S820: 10,12; THO1: 7; TPOX: 8,11; vWA: 16,17