Disease:

 Glioblastoma (Multiform) 

Origin:

 Undifferentiated glioblastoma 

Species:

 Homo sapiens 

Tissue:

 CNS 

Properties:

 Adherent 

Cytogenic data:

 5n+/-, Near-pentaploid 115+/- (104-126) 

Patient:

 Female, 67 yrs of age 

Medium:

 RPMI1640 + 10% FBS 

Subculture:

 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Mutations in SF-295 Cells 


References for SF-295 cells:

1. Rutka JT, Giblin JR, Dougherty DY, Liu HC, McCulloch JR, Bell CW, Stern RS, Wilson CB, Rosenblum ML. Establishment and characterization of five cell lines derived from human malignant gliomas. Acta Neuropathol. 1987;75(1):92-103.