分子量:56.8 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human ALCAM, His Tag by the LAL method.

Buffer:PBS, pH 7.4

生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human ALCAM, His Tag at 10 μg/mL (100 μl/well) can bind mouse CD6 Fc Chimera, The EC50 of mouse CD6 Fc Chimera is 0.1-0.22 μg/mL.

产品特性:Human ALCAM, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 56.8 kDa. The predicted N-terminus is Trp 28. The reducing (R) protein migrates as 65-80 kDa in SDS-PAGE  due to glycosylation.

产品背景:CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.