大鼠巨噬细胞Cell Specification
Macrophages are cells produced by the differentiation of monocytes in tissues. Macrophages are
large cells found within many types of tissue. They derive from monocytes that have entered
tissues. Macrophages phagocytise invading microorganisms and also scavenge dead and
damaged cells and cellular debris. In the bone marrow and subsequestly in the blood and tissues,
cells of this series undergo a series of functional and morphologic maturation steps that
culminate in the mature tissue macrophage [1]. Macrogphage can be identified by specific
expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human),
MAC-1/MAC-3 and CD68 by flow cytometory or immunohistochemical staining [2].
RMa-bm from ScienCell Research Laboratories are isolated from adult rat bone marrow tissue.
Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in
1 ml volume. RMa-bm is characterized by immunofluorescent method with antibody to CD 11b.
RMa-bm is negative for mycoplasma, bacteria, yeast and fungi. RMa-bm is guaranteed to further
culture in the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. No. 1921) for the culturing of RMabm
in vitro.
大鼠巨噬细胞Product Use
RMa-bm are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional
Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69.
[2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews
Immunology 5(12) 953-964.
ScienCell
Research Laboratories
TM
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the order:
1. Macrophages are not expected to further expand in culture. It is recommended to use
either cell culture-grade or bacterial-grade plastics for the culturing of macrophages since
they easily attach to culture plastics.
2. Prepare complete medium: decontaminate the external surfaces of medium and medium
supplements with 70% ethanol and transfer them to sterile field. Aseptically open each
supplement tube and add them to the basal medium with a pipette. Rinse each tube with
medium to recover the entire volume.
3. Place the vial in a 37oC waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, rinse
the vial with 70% ethanol and transfer it to a sterile field. Remove the cap, being careful
not to touch the interior threads with fingers. Using a 1 ml eppendorf pipette gently resuspend
the contents of the vial.
4. Transfer the contents of the vial into a 15 ml centrifuge tube which contains 10 ml of
macrophage medium. Centrifuge the tube at 1000rpm for 5 min.
大鼠巨噬细胞5. Discard the supernatant; resuspend the cell pellet in macrophage medium and plate cells
in the flask or plate.
6. Return the culture vessels to the incubator.
7. For best result, do not disturb the culture for at least 16 hours after the culture has been
initiated. Change the growth medium the next day to remove the residual DMSO and
unattached cells, then every other day thereafter. A health culture will display polygonal
shaped, sheets of contiguous cells and the cell number will be double after two to three
days in culture.
Maintenance of Culture:
1. Change the medium to fresh supplemented medium the next morning after establishing a
culture from cryopreserved cells.
大鼠巨噬细胞2. Change the medium every two to three days thereafter.
It is not recommended that macrophage be subcultured beyond their initial plating.
Caution: Handling animal derived products is potentially biohazardous. Always wear gloves and safety glasses
when working these materials. Never mouth pipette. We recommend following the universal procedures for
handling products of human origin as the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).