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Mouse Roof plate-specific Spondin 1 / R-Spondin 1 Protein
 
 
 
 
 
 
 
Mouse RSPO1 / R-Spondin-1 Protein Price Inquiry ( Available Sizes )
 
 
 
500μg:  询价;
 
≥1mg 大包装:  询价
 
 
 
Mouse RSPO1 / R-Spondin-1蛋白产品信息
 
 
 
 
别名:
 
 
RP23-325M14.2,  R-spondin,  Rspondin
 
 
 
 
表达方式:
 
 
A DNA sequence encoding the full length of mouse RSPO1 ( NP_619624.2 ) ( Met 1 - Gln 265 ) was fused with a polyhistidine tag at the C-terminus
 
 
 
 
种属:
 
Mouse
 
 
 
表达宿主: 
 
Human Cells
 
 
 
 
Mouse RSPO1 / R-Spondin-1蛋白质控指标
 
 
 
 
纯度:
 
> 80 % as determined by SDS-PAGE
 
SDS-PAGE:
 
 
R-Spondin-1 protein
 
 
 
 
内毒素:
 
< 1.0 EU per μg of the protein as determined by the LAL method
 
 
 
稳定性:
 
Samples are stable for up to twelve months from date of receipt at -70℃
 
 
 
预测N端:
 
Ser 21
 
 
 
分子量:
 
 
The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions
 
 
 
 
缓冲液:
 
Lyophilized from sterile PBS , pH 7.4 
 
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
 
Please contact us for any concerns or special requirements.
 
 
 
 
 
 
Mouse RSPO1 / R-Spondin-1蛋白使用指南
 
 
 
 
储存方法:
 
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
 
 
 
 
 
Reconstitution:
 
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 
 
 
 
Mouse RSPO1 / R-Spondin-1蛋白相关产品和研究主题
 
 
相关领域:
 
Proteins:
 
 
 
 
Molecule
 
Species
 
Description //For Detailed Info. and Price------CLICK!
 
Cat No
 
 
 
RSPO1
 
Human
 
RSPO1 Protein, Recombinant 
 
11083-H08H
 
 
 
RSPO1
 
Human
 
RSPO1 (aa 1-146) Protein, Recombinant
 
11083-H08H1
 
 
 
RSPO1
 
Mouse
 
RSPO1 Protein, Recombinant
 
50316-M08H
 
 
 
 
Antibodies:
 
 
 
 
Molecule
 
Application
 
Description //For Detailed Info. and Price------CLICK!
 
Cat No
 
 
 
Human
 RSPO1
 
WB
 
RSPO1 Antibody, Rabbit MAb
 
11083-R010
 
 
 
Human
 RSPO1
 
ELISA
 
RSPO1 Antibody, Rabbit MAb
 
11083-R106
 
 
 
Human 
 RSPO1
 
WB, ELISA
 
Rabbit Polyclonal Antibody
 
11083-RP01
 
 
 
Human 
 RSPO1
 
WB, ELISA
 
Rabbit Polyclonal Antibody (Antigen Affinity Purified)
 
11083-RP02
 
 
 
 
 
Mouse RSPO1 / R-Spondin-1蛋白背景综述
 
Mouse R-Spondin 1 ( Roof plate-specific Spondin 1 ), also known as RSPO1 and Cristin 3, is a secreted protein which belongs to the R-Spondin family. The R-Spondin (RSPO) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. R-Spondin1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1 / Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. RSPO1 contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. Mouse R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with human R-Spondin1. RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY.
 
参考文献
 
 
 
Parma, P. et al., 2006, Nature genetics. 38 (11): 1304-9.
 
Capel, B., 2006, Nature genetics. 38 (11) :1233-4.
 
Binnerts, ME. et al., 2007, Proc Natl Acad Sci. 104 (37): 14700-5.
 
Wilhelm, D., 2007, Bioessays.  29 (4): 314-8.
 
Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6.