牛非神经元性烯醇化酶(ENO1)多克隆抗体
Polyclonal Antibody to Enolase 1 (ENO1)
[PRODUCT INFORMATION]
Immunogen: NNE, Bovine
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant NNE (Met1~Lys434) expressed in E.coli. USCN 
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-MSILKVHARE IFDSRGNPTV EVDLFTAKGL FRAAVPSGAS
TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVVEQEKI DKLMIEMDGT
ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN AEVILPVPAF NVINGGSHAG
NKLAMQEFMI LPVGAENFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN
KEALELLKNA IGKAGYSDKV VIGMDVAASE FYRSGKYDLD FKSPDDPSRY ITPDELANLY
KSFIRDYPVV SIEDPFDQDD WEAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVSEKSCNCL
LLKVNQIGSV TESLQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTVAPCR
SERLAKYNQI LRIEEELGSK AKFAGRSFRN PLAK
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against NNE. It has beenselected for its ability to recognize NNE in immunohistochemical staining andwestern blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user
[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[QUALITY CONTROL ] 
Content:The quality control contains recombinant NNE (Met1~Lys434)disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.


本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。