分子量:41.6 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Cathepsin E, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2.

产品特性:Human Cathepsin E, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 41.6 kDa. The predicted N-terminus is Ser 20 (pro) or Ile 54 (mature). The reducing (R) protein migrates as 40-46 kDa in SDS-PAGE  due to glycosylation.

产品背景:Cathepsin E (CTSE) is also known as Cd152,Ly56 and Ctla4,which can be cleaved into the following 2 chains: Cathepsin E form I and Cathepsin E form II. Cathepsin E belongs to the peptidase A1 family. CTSE is similar to cathepsin D, but slightly broader specificity. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. CTSE is expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen, but not expressed in resting B-lymphocytes. CTSE may have a role in immune function,activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.At the same time, CTSE is also probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation.