钙网蛋白(CRT)重组蛋白

YB486Ra011
Recombinant Calreticulin (CRT)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
钙网蛋白(CRT)重组蛋白Residues: Glu18~Ser193
Tags: N-terminal His-Tag
Accession: P27797
Host: E. coli
Subcellular Location: Endoplasmic reticulum lumen.
Cytoplasm, cytosol. Secreted, extracellular space,
extracellular matrix. Cell surface. Sarcoplasmic
reticulum lumen. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
钙网蛋白(CRT)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 10mM
PBS, pH7.4, containing 1mM DTT, 5% trehalose,
0.01% sarcosyl and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 21.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
钙网蛋白(CRT)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
EPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE EKDKGLQTSQ
DARFYALSAS FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPNSLDQT DMHGDSEYNI
MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VES
[ REFERENCES ]
1. McCauliffe D.P., et al. (1990) J. Clin. Invest. 85:1379-1391.
2. McCauliffe D.P., et al. (1992) J. Biol. Chem. 267:2557-2562.
3. Goshima N., et al. (2008) Nat. Methods 5:1011-1017.
4. Lieu T.-S., et al. (1988) J. Clin. Invest. 82:96-101.