分子量:20.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human PLGF, His Tag by the LAL method.

Buffer:100 mM acetic acid, pH2-3

生物活性:Measured in a cell proliferation assay using MDA - MB  231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 g/mL.

产品特性:Human PLGF, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. The reducing (R) protein migrates as 28-33 kDa in SDS-PAGE  due to glycosylation.

产品背景:Placental growth factor (PGF) is also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.