GelRed 核酸凝胶染料!EB替代产品!

来源: 北京方程生物科技有限公司   2013-12-5   访问量:4918评论(0)

GelRed

欢迎致电北京方程生物 010-57266903

Biotium公司的GelRed GelGreen 无论用于预制凝胶染色还是凝胶电泳后染色,都表现出了极高的灵敏度。为配合 312 nm UV 凝胶成像系统而设计的 GelRed ,在使用了我们新开发的具有专利的试验步骤后(该试验步骤中使用稀释的 NaCl 溶液替代传统的 TBE 缓冲溶液),在凝胶电泳后染色中优于或者至少相当于 SYBR Gold 。但与 SYBR Gold 不同, GelRed 在预制凝胶中使用时仍然有极高的灵敏度,事实上GelRed 在检测低浓度、微量 DNA 方面比 EB 表现出更高的灵敏度,尤其对小分子量的 DNA 检测非常灵敏(图1)。 GelGreen 可以满足使用 488 nm 激光凝胶扫描仪或者可见光激发的 Dark Reader 的研究人员的使用要求。 GelGreen 无论是在预制凝胶还是凝胶电泳后染色中都与 SYBR Green I 灵敏度相当,但不存在后者的不稳定性问题。实际上, GelRed 和 GelGreen ,特别是 GelRed 非常稳定,可以长期在室温下保存。当这两种染料稀释在 TBE 或者类似的电泳缓冲溶液中时甚至可以使用微波炉加热,便于采用常规方法制备预制凝胶。含有染料的预制凝胶可以成批制备,长期保存。GelRed

Product Description

GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed™ is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed™ and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed™ without changing your existing imaging system.

GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.

A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed/GelGreen Safety Report.

For new users, Biotium recommends GelRed™ 10,000X solution in water (41003), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRed™ 10,000X solution in DMSO for established users of GelRed™ in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRed™ 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRed™ 3X in water (41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10 mL.

FEATURES

Safer than EB: Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash
Ultra-sensitive: Much more sensitive than EtBr and SYBR Safe
Extremely stable: Available in water, stable at room temperature for long-term storage and microwavable
Simple to use: Very simple procedures for either precast and post gel staining
Perfectly compatible with a standard UV transilluminator : GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
Perfectly compatible with downstream applications : Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
The Most Sensitive and Stable Precast Gel StainFigure 1.GelRed™ is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.
The Most Sensitive and Stable Post Gel StainFigure 2. GelRed™ displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRed™and SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.



同样重要的是, GelRed 和 GelGreen 相比 EB 或 SYBR Green I 提高了安全性。 独立的测试服务公司( Litron Laboratories, Inc. )进行的标准艾姆斯氏测试结果表明, 在 18.5 μ g /mL (该浓度远高于推荐用于电泳后染色的 约 4 μ g/mL 的 3X 染色液 )浓度下, GelGreen 没有诱变性,而 GelRed 也仅在 S9 代谢活化时有微弱的诱变性。 GelRed 和 GelGreen 的特殊化学结构使其难以穿透细胞膜进入细胞,正是这一特性降低了染料的细胞毒性。如图3。相反, SYBR Green I 广泛地用于活细胞线粒体和核 DNA 染色,这正是由于它能快速的被细胞吞入。由于已知 SYBR Green I 对紫外线导致的突变有强烈的增强作用( Ohta et al. Mutat. Res. 492 , 91(2001) ),因此它的高细胞膜透性使它在紫外环境观察时成为凝胶染色的主要有害物质。
GelRed


11745832910 I型高效地高辛 ROCHE

11585614910 II型高效地高辛 ROCHE

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