Product Highlight

• Configuration-specific monoclonal antibody that recognizes Rab11-GTP, but not Rab11-GDP
• The only available Rab11 activation assay kit directly measures the level of Rab11-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Rab11 in cells and in tissues by immunohistochemistry

Product Description

Small GTPases are a super-family of cellular signaling regulators. Rab11 has been shown to control traffic through the recycling endosome.

Currently there is no direct assay to measure the activation of Rab GTPases.

NewEast Biosciences Rab11 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab11-GTP, but not Rab11-GDP, and a Rab11 specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

This anti-Rab11-GTP monoclonal antibody can also be used to monitor the activation of Rab11 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Rab11 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab11-GTP, but not Rab11-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

 

Assay Principle

NewEast Biosciences Rab11 Activation Assay Kit bases on the configuration-specific anti- Rab11-GTP monoclonal antibody to measure the active Rab11-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab11 activation assays. Briefly, anti-active Rab11 mouse monoclonal antibody will be incubated with cell lysates containing Rab11-GTP. The bound active Rab11 will then be pulled down by protein A/G agarose. The precipitated active Rab11 will be detected by immunoblot analysis using an anti-Rab11 rabbit polyclonal 

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance     Endocrinology. 2014 Sep;155(9):3315-28