Product Highlight

• Configuration-specific monoclonal antibody that recognizes Rab35-GTP, but not Rab35-GDP
• The only available Rab35 activation assay kit directly measures the level of Rab35-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry

Product Description

The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.

Currently there is no direct assay to measure the activation of Rab35 GTPases.

NewEast Biosciences Rab35 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab35-GTP, but not Rab Rab35-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti- Rab35-GTP monoclonal antibodies can also be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Rab35 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab35. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Rab35 Activation Assay Kit bases on the configuration-specific anti-Rab35-GTP monoclonal antibody to measure the active Rab35-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab35 activation assays. Briefly, anti-active Rab35 mouse monoclonal antibody will be incubated with cell lysates containing Rab35-GTP. The bound active Rab35 will then be pulled down by protein A/G agarose. The precipitated active Rab35 will be detected by immunoblot analysis using anti- Rab35 rabbit polyclonal antibody.

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance     Endocrinology. 2014 Sep;155(9):3315-28