Anti-RAS (A146V) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-RAS (A146V) Mouse Monoclonal Antibody

Anti-RAS (A146V) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-RAS (A146V) Mouse Monoclonal Antibody

英文名称： Anti-RAS (A146V) Mouse Monoclonal Antibody

产品编号： 26479

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

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Ras (A146V) [26479]
[100ul]		  

 
Catalog Number:  26479
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-RAS (A146V) Mouse Monoclonal Antibody
Background:   The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A146V mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant Ras (A146V) and wild-type proteins. Purified His-tagged Ras (A146V) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-Ras (A146V) monoclonal antibody (Cat. #26479).
Immunofluorescence:  
Immunofluorescence of cells expressing KRas proteins with anti-KRas (A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (A146V) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146P) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRas (A146V) monoclonal antibody (Cat. #26479).
Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136