Neuroglycan C,跨膜硫酸软骨素蛋白聚糖C抗体

产品编号HZ-11323R
英文名称Neuroglycan C
中文名称跨膜硫酸软骨素蛋白聚糖C抗体
别 名Acidic leucine rich EGF like domain containing brain protein; Acidic leucine-rich EGF-like domain-containing brain protein; Caleb; CSPG5_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 干细胞 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
Neuroglycan C,跨膜硫酸软骨素蛋白聚糖C抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Neuroglycan C
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Neuroglycan C,跨膜硫酸软骨素蛋白聚糖C抗体PubMedPubMed
产品介绍background:
Neuroglycan C is a brain-specific chondroitin sulfate proteoglycan (CSPG) implicated in the proliferation of neural stem and progenitor cells. Neuro-glycan C is a single-pass membrane protein that can manifest as a part-time proteoglycan depending on the tissue expressing it. In its proteoglycan form, Neuroglycan C exhibits chondroitin sulfate glycans and functions as a receptor for midkine, a growth factor that binds heparin, to affect cytoskeletal changes. By means of ectodomain shedding, the ectodomain of Neuroglycan C is able to enhance neurite outgrowth from neurons. Neurite growth stimulation is affected by both an EGF-like and an acidic amino acid domain found on the shed ectodomain. Both domains instigate neurite growth, however, these domains exhibit differing functionality as to number of neurites produced and neuron types stimulated.

Function:
May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.

Subunit:
Binds TNR and probably TNC (By similarity). Interacts with ERBB3 and GOPC.

Subcellular Location:
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts.

Tissue Specificity:
Restricted to brain (at protein level).

Post-translational modifications:
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation.
Phosphorylated; in intracellular and extracellular parts.

Neuroglycan C,跨膜硫酸软骨素蛋白聚糖C抗体Similarity:
Contains 1 EGF-like domain.

Gene ID:
10675

Database links:
Entrez Gene: 10675 Human
Entrez Gene: 29873 Mouse
Entrez Gene: 50568 Rat
Omim: 606775 Human
SwissProt: O95196 Human
SwissProt: Q49AA8 Human
SwissProt: Q71M36 Mouse
SwissProt: Q9ERQ6 Rat
Unigene: 45127 Human
Unigene: 38496 Mouse
Unigene: 10146 Rat

Neuroglycan C,跨膜硫酸软骨素蛋白聚糖C抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片	(human/cell line)U251 lysates probed with Neuroglycan C Polyclonal Antibody, Unconjugated (Catalog #HZ-11323R) at 1:300 overnight at 4ⅹC. Followed by a conjugated secondary antibody (Secondary Catalog #926-32211) at 1:10000 for 60 min at 37ⅹC.