Elastin,项韧带弹性蛋白抗体

产品编号HZ-11057R
英文名称Elastin
中文名称项韧带弹性蛋白抗体
别 名Elastin; ELN; SVAS; Tropoelastin; WBS; WS.ELN_BOVIN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞粘附分子 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow,
Elastin,项韧带弹性蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量80kDa
细胞定位细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from bovine Elastin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Elastin,项韧带弹性蛋白抗体PubMedPubMed
产品介绍background:
Elastic fibers, which are comprised primarily of elastin, endow loose connective tissue with a resilience that compliments the tensile strength of collagenous fibers. Elastin is the main component of the extracellular matrix of arteries, and it performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. Elastin is composed largely of glycine, proline, and other hydrophobic residues, and it contains multiple lysine-derived crosslinks, such as desmosines, which link individual polypeptide chains into a rubber-like network. During aging, the elasticity of connective tissue becomes reduced because of the cross-linking of collagenous fibers with elastin. Deficiencies of elastin are associated with multiple disorders, such as supravalvular aortic stenosis and Williams-Beuren syndrome. The human elastin gene maps to chromosome 7q11.23.

Function:
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.

Subunit:
The polymeric elastin chains are cross-linked together into an extensible 3D network. Forms a ternary complex with BGN and MFAP2. Interacts with MFAP2 via divalent cations (calcium > magnesium > manganese) in a dose-dependent and saturating manner.

Subcellular Location:
Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.

Elastin,项韧带弹性蛋白抗体Post-translational modifications:
Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

Similarity:
Belongs to the elastin family.

Database links:
Entrez Gene: 2006 Human
Entrez Gene: 25043 Rat
Omim: 130160 Human
SwissProt: P15502 Human
SwissProt: P54320 Mouse
SwissProt: Q99372 Rat
Unigene: 647061 Human
Unigene: 275320 Mouse
Unigene: 404771 Mouse
Unigene: 54384 Rat

Elastin,项韧带弹性蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated(HZ-11057R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated(HZ-11057R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining