DDX23,三磷酸腺苷依赖解旋酶DDX23抗体

产品编号HZ-12199R
英文名称DDX23
中文名称三磷酸腺苷依赖解旋酶DDX23抗体
别 名100 kDa U5 snRNP-specific protein; DEAD box protein 23; EC 3.6.1.-; PRP28 homolog; U5-100kD; DDX23_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
DDX23,三磷酸腺苷依赖解旋酶DDX23抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量96kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DDX23
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DDX23,三磷酸腺苷依赖解旋酶DDX23抗体PubMedPubMed
产品介绍background:
DDX23 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Function:
Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation.

Subunit:
The phosphorylated form (by SRPK2) associates wth tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins). Identified in the spliceosome C complex. Interacts with ERBB4.

Subcellular Location:
Nuclear

DDX23,三磷酸腺苷依赖解旋酶DDX23抗体Post-translational modifications:
In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Phosphorylated by SRPK2 and this phosphorylation is required for its association with the tri-snRNP (U4/U6-U5 tri-small nuclear ribonucleoproteins) and subsequent spliceosomal B complex formation. [SIMILARITY] Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.

Similarity:
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

Database links:
UniProtKB/Swiss-Prot: Q9BUQ8.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.