CysLT1,半胱氨酰白三烯受体1型抗体

产品编号HZ-14168R
英文名称CysLT1
中文名称半胱氨酰白三烯受体1型抗体
别 名CLTR1_HUMAN; CYSLT 1; CysLT(1); CYSLT1R; CYSLTR 1; CYSLTR; CysLTR vide supra; CYSLTR1; Cysteinyl leukotriene D4 receptor; Cysteinyl leukotriene receptor 1; G-protein coupled receptor HG55; HG55; HMTMF81; LTD4 receptor; MGC46139.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 G蛋白偶联受体 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
CysLT1,半胱氨酰白三烯受体1型抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CYSLTR1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CysLT1,半胱氨酰白三烯受体1型抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Function:
Receptor for cysteinyl leukotrienes mediating bronchoconstriction of individuals with and without asthma. Stimulation by LTD4 results in the contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

Subcellular Location:
Cell membrane.

Tissue Specificity:
Widely expressed, with highest levels in spleen and peripheral blood leukocytes. Lower expression in several tissues, such as lung (mostly in smooth muscle bundles and alveolar macrophages), placenta, small intestine, pancreas, colon and heart.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Gene ID:
10800

CysLT1,半胱氨酰白三烯受体1型抗体Database links:
Entrez Gene: 10800 Human
Entrez Gene: 58861 Mouse
Omim: 300201 Human
SwissProt: Q9Y271 Human
SwissProt: Q99JA4 Mouse
Unigene: 201300 Human
Unigene: 287166 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CysLT1 Polyclonal Antibody, Unconjugated(HZ-14168R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining