Streptavidin polyclonal antibody
产品名称： Streptavidin polyclonal antibody
英文名称： Streptavidin polyclonal antibody
产品编号： PAB24945
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against native streptavidin.
- Immunogen:
- Native purified streptavidin from Streptomyces avidinii.
- Host:
- Rabbit
- Form:
- Liquid
- Purification:
- Ion exchange chromatography purification
- Storage Buffer:
- In 20 mM K3PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:200000)
Immunohistochemistry (1:1500-1:5000)
Western Blot (?:3000-1:12000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Recombinant protein)
- Streptavidin and Biotin conjugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of Streptavidin polyclonal antibody (Cat # PAB24945). Detection shown was using Donkey anti Rabbit (Dylight 649) (1:10, 000 1.5 hr RT in blocking buffer) and imaged on the BioRad VersaDoc System.