Anti-IDH2 (R172S) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-IDH2 (R172S) Mouse Monoclonal Antibody

Anti-IDH2 (R172S) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-IDH2 (R172S) Mouse Monoclonal Antibody

英文名称： Anti-IDH2 (R172S) Mouse Monoclonal Antibody

产品编号： 26408

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

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IDH2 (R172S) [26408]
[100ul]		  

Catalog Number:  26408
Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Description:   Anti-IDH2 (R172S) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172S IDH2 mutations do exist in diffusely infiltrative gliomas.
Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172S, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes R172S mutant, but not wild-type IDH2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant IDH2 (R172S) and wildtype proteins. Purified His-tagged IDH2 (R172S) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with anti-IDH2 (R172S) monoclonal antibody (Cat. #26408).
Immunofluorescence:  
Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172S) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (R172S) plasmid, pCDNA3-GFP-IDH2 (WT) plasmid, pCDNA3-GFP-IDH2 (R172G) plasmid, pCDNA3-GFP-IDH2 (R172K) plasmid, pCDNA3-GFP-IDH2 (R172W) plasmid or pCDNA3-GFP-IDH2 (R172H) plasmid, then fixed and stained with anti-IDH2 (R172S) monoclonal antibody (Cat. #26408)
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015