Disease:

 Lung carcinoma 

Origin:

 Established from the lung carcinoma of a 52-year-old Caucasian man 

Species:

Homo sapiens 

Tissue:

Lung 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

hypotriploid to hypertriploid with abnormalities 

Patient:

Male, Caucasian, 52 yrs of age 

Medium:

DMEM/F12 + 10% FBS 

Subculture:

1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Isoenzymes:
 AK-1, 2; ES-D, 1; G6PD, B; GLO-I, 1; PGM1, 1-2; PGM3, 1 
DNA Profile (STR):
 Amelogenin: X,Y; CSF1PO: 10,12; D13S317: 11,12; D16S539: 11,13; D5S818: 12; D7S820: 8,12; THO1: 9; TPOX: 8,11; vWA: 17 
Related cells by Organ:

A427 cells 

A549 cells 

Calu3 cells 

H460 cells 


References for A427 cells:

1. Lu, X. and Arthur, G. The differential susceptibility of A427 and A549 cell lines to the growth-inhibitory effects of ET-18-OCH3 does not correlate with the relative effects of the alkyl-lysophospholipid on the incorporation of fatty acids into cellular phospholipids. Cancer Res, 52: 2813-2817, 1992.