DDX4 polyclonal antibody
产品名称： DDX4 polyclonal antibody
英文名称： DDX4 polyclonal antibody
产品编号： PAB2479
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DDX4.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DDX4.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia.
Guo X, Gui YT, Tang AF, Lu LH, Gao X, Cai ZM.Asian J Androl. 2007 May;9(3):339-44.
- 2.
- VASA is a specific marker for both normal and malignant human germ cells.
Zeeman AM, Stoop H, Boter M, Gillis AJ, Castrillon DH, Oosterhuis JW, Looijenga LH.Lab Invest. 2002 Feb;82(2):159-66.
- 3.
- The human VASA gene is specifically expressed in the germ cell lineage.
Castrillon DH, Quade BJ, Wang TY, Quigley C, Crum CP.Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9585-90.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of DDX4 polyclonal antibody (Cat # PAB2479) in K-562 cell line lysates (35 ug/lane). DDX4 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 polyclonal antibody (Cat # PAB2479) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 54514
- Protein Accession#:
- NP_077726;Q9NQI0
- Gene Name:
- DDX4
- Gene Alias:
- MGC111074,VASA
- Gene Description:
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
- Omim ID:
- 605281
- Gene Ontology:
- Hyperlink
- Gene Summary:
- DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq
- Other Designations:
- DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4,OTTHUMP00000122546