Description:
A reliable method for the proteolytic digestion of proteins in gel for subsequent analysis by mass spectrometry.The protein spots are first excised from the gel and transferred to a proteomic grade tube . Silver stained gel pieces are washed with SilverOUT™ to remove inhibitory silver ions and then the gel pieces are treated with our proprietary Trypsin-Digestion Buffer Mix, a mixture of MSG-Trypsin™ and an optimal digestion buffer, which ensures reliable and efficient protein digestion.The resulting digested peptides are extracted with Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without any subsequent treatments or cleaning procedures.The InGel™ kit is supplied with all the necessary reagents for 100 protein spots and includes:SilverOUT™ destaining reagent. MSG-Trypsin™, a mass spectrometry grade trypsin with minimal autolysis and increases stability. Trypsin Digestion Buffer for optimal trypsin activity. Pep-Extract™ Buffer, for high level peptide extraction. Store: 18～25℃
Features:
Supplied with a mass spectrometry grade  trypsin;Suitable for digestion and extraction of 100 protein spots;Quality tested for mass spectrometry applications;Suitable for 2D gels stained with silver stain, Coomassie dye, and other methods