Proteasome 20S alpha 2,蛋白酶体PSMα2抗体

产品编号HZ-9351R
英文名称Proteasome 20S alpha 2
中文名称蛋白酶体PSMα2抗体
别 名20S Proteasome α2; HC3; Macropain subunit C3; MU antibody Multicatalytic endopeptidase complex subunit C3; PMSA2; Proteasome (prosome macropain) subunit alpha type 2; Proteasome alpha 2 subunit; Proteasome component C3; Proteasome subunit alpha type 2; Proteasome subunit alpha type-2; Proteasome subunit HC3; PSC2; PSC3; PSMA 2; psmA2; PSA2_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep,
Proteasome 20S alpha 2,蛋白酶体PSMα2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量26kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Proteasome 20S alpha 2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Proteasome 20S alpha 2,蛋白酶体PSMα2抗体PubMedPubMed
产品介绍background:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.

Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.

Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Subcellular Location:
Cytoplasm. Nucleus. Cytoplasm, P-body (By similarity). Note=Co-localizes with TRIM5 in the cytoplasmic bodies (By similarity).

Post-translational modifications:
Phosphorylated on tyrosine residues; which may be important for nuclear import (By similarity).

Proteasome 20S alpha 2,蛋白酶体PSMα2抗体Similarity:
Belongs to the peptidase T1A family.

Gene ID:
5683

Database links:
Entrez Gene: 5683 Human
Entrez Gene: 19166 Mouse
Entrez Gene: 29669 Rat
Omim: 176842 Human
SwissProt: P25787 Human
SwissProt: P49722 Mouse
SwissProt: P17220 Rat
Unigene: 333786 Human
Unigene: 252255 Mouse
Unigene: 1617 Rat

Proteasome 20S alpha 2,蛋白酶体PSMα2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PSA2) Polyclonal Antibody, Unconjugated (HZ-9351R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.