FITC标记的磷酸化嗜中性粒细胞胞浆因子1抗体
产品名称： FITC标记的磷酸化嗜中性粒细胞胞浆因子1抗体
英文名称： Anti-phospho-NCF1 (Ser359)/FITC
产品编号： HZ-11445R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-NCF1 (Ser359)/FITC Conjugated antibody
FITC标记的磷酸化嗜中性粒细胞胞浆因子1抗体
| 产品编号 | bs-11445R-FITC |
| 英文名称 | Anti-phospho-NCF1 (Ser359)/FITC |
| 中文名称 | FITC标记的磷酸化嗜中性粒细胞胞浆因子1抗体 |
| 别 名 | phospho-NCF1(Ser359); phospho-p47 phox(Ser359); NCF1 (phospho S359); 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane. Function: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Subunit: Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity. DISEASE: Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Similarity: Contains 1 PX (phox homology) domain. Contains 2 SH3 domains. Database links: Entrez Gene: 281345 Cow Entrez Gene: 653361 Human Entrez Gene: 17969 Mouse Entrez Gene: 100134857 Pig Entrez Gene: 100008803 Rabbit Entrez Gene: 114553 Rat Omim: 608512 Human SwissProt: O77774 Cow SwissProt: P14598 Human SwissProt: Q09014 Mouse Unigene: 647047 Human Unigene: 655201 Human Unigene: 425296 Mouse Unigene: 38575 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
遗传性慢性肉芽肿性疾病（CGF）与P47 PHX和p67 PHX基因突变有关。细胞质蛋白P47 PHOX和p67 PHOX，也被指定为中性粒细胞胞浆因子（NCF）1和NCF2，在中性粒细胞和其他吞噬细胞中激活超氧化物产生的NADPH氧化酶是必需的。在激活NADPH氧化酶时，P47 PHOX和p67 PHX迁移到质膜上，它们与细胞色素b55 8和小G蛋白RAC结合形成功能性酶复合物。P47 PHX和p67 PHX均含有两个Src同源性3（SH3）结构域。P67 PHX的C-末端SH3结构域已被证明与P47 PHOX的富含脯氨酸结构域相互作用，提示p47 PHOX可使p67 PHOX转运至膜。