MAdCAM1,粘膜细胞粘附分子1抗体

产品编号HZ-11179R
英文名称MAdCAM1
中文名称粘膜细胞粘附分子1抗体
别 名Addressin mucosal; hMAdCAM 1; hMAdCAM-1; hMAdCAM1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1.
说 明 书0.1ml 0.2ml
研究领域心血管 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
MAdCAM1,粘膜细胞粘附分子1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human MAdCAM1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
MAdCAM1,粘膜细胞粘附分子1抗体PubMedPubMed
产品介绍background:
The recirculation of lymphocytes through different organs is thought to be regulated by adhesion molecules recognizing tissue-specific vascular addressins on the endothelium. The mucosal vascular addressin, MadCAM-1 (mucosal addressin cell adhesion molecule 1), is an immunoglobulin superfamily adhesion molecule for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into Peyer’s patches and the intestinal lamina propria. MadCAM-1 acts as an endothelial cell ligand for leukocyte homing receptors L-Selectin and Integrin Alpha 4/Beta 7. MadCAM-1 is strongly expressed on inflamed portal vein/sinusoidal endothelium in autoimmune-mediated liver disease and plays a major contributory role in the progression of chronic experimental autoimmune encephalomyelitis.

Function:
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Subunit:
Contains 2 Ig-like (immunoglobulin-like) domains.

Subcellular Location:
Membrane.

Tissue Specificity:
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

Post-translational modifications:
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation (By similarity).

MAdCAM1,粘膜细胞粘附分子1抗体Similarity:
Contains 2 Ig-like (immunoglobulin-like) domains.

Database links:
UniProtKB/Swiss-Prot: Q13477.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-MAdCAM1 Polyclonal Antibody, Unconjugated(HZ-11179R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining