LightCycler® Uracil-DNA Glycosylase  

双杂交探针®尿嘧啶-DNA 糖基化酶

For general laboratory use.

供一般实验室使用。

Product No：03539806001   产品编号：03539806001  

Pack Size：100 U (100 reactions)  包装尺寸：100 U（100 次反应）

Keywords: Other qPCR Consumables  关键词：其他 qPCR 耗材

Overview概述：

LightCycler® Uracil-DNA Glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and double-stranded DNA, excising uracil and creating alkali- and heat-sensitive abasic sites in the DNA.

LightCycler ® 尿嘧啶-DNA糖基化酶水解单链和双链DNA中U-DNA位点的尿嘧啶-糖苷键，切除尿嘧啶并在DNA中产生对碱和热敏感的脱碱基位点。

The enzyme is more active on single-stranded DNA than on double- stranded DNA.

该酶对单链 DNA 的活性高于对双链 DNA 的活性。

The enzyme is inactive on RNA and native, uracil-free DNA.

该酶对 RNA 和天然无尿嘧啶 DNA 无活性。

Contents 内容

The enzyme is supplied in storage buffer at 2 U/μl.

该酶以 2 U/μl 的浓度提供在储存缓冲液中。

Application应用：

LightCycler® Uracil-DNA Glycosylase (LightCycler® UNG) is ideally suited for preventing carryover contamination between PCRs. This technique involves incorporating deoxyuridine triphosphate (dUTP) into all amplification reactions, and pretreating all successive LightCycler® FastStart Enzyme-based PCR mixtures with LightCycler® UNG. Prior to the thermal-cycling procedure, LightCycler® UNG is added to the LightCycler® amplification reaction mixture, then incubated for 10 minutes at +40°C. During the initial activation of FastStart DNA Polymerase (+95°C for 10 minutes), the LightCycler® UNG becomes inactive.

LightCycler ® 尿嘧啶-DNA 糖基化酶 (LightCycler ®  UNG) 非常适合防止 PCR 之间的残留污染。该技术包括将脱氧尿苷三磷酸 (dUTP) 加入所有扩增反应中，并使用 LightCycler ®  UNG预处理所有连续的基于LightCycler ®  FastStart 酶的 PCR 混合物。在热循环程序之前，将 LightCycler ®  UNG 添加到 LightCycler ® 扩增反应混合物中，然后在 +40°C 下孵育 10 分钟。在 FastStart DNA 聚合酶的初始激活期间（+95°C 10 分钟），LightCycler ®  UNG 变得不活跃。

Note: LightCycler® UNG is designed only for use with the FastStart Enzyme or FastStart Enzyme-based LightCycler® Kits.

注意： LightCycler®  UNG 仅设计用于 FastStart 酶或基于 FastStart 酶的 LightCycler ® 试剂盒。