The 5X reaction mix contains a reaction buffer supplemented with dNTPs and is optimized for robust multiple target detection in a simple workflow. The 25X enzyme mix features Luna WarmStart Reverse Transcriptase and Q5 Hot Start High-Fidelity DNA
Polymerase. This dual-temperature control of enzyme activities enables room temperature reaction setup; preassembled reactions are stable at room temperature for up to 24 hours. The kit has robust multiplex target amplification capacity and enables various applications such as diagnostics, pathogen detection, and viral genome sequencing (including the ~50 amplicons per reaction used in ARTIC SARS-CoV-2 sequencing protocols).