This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900 II SFM features:

 Superior long-term, high-density growth
 Optimized for recombinant protein production
 Serum-free, protein-free, ready-to-use formulation
 Scalable in bioreactors

Superior long-term, high-density growth:
Spodoptera frugiperda (Sf9) cells grown in Sf-900 II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900 II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production:
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900 II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation:
Sf-900 II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900 II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900 II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors:
The utility of Sf-900 II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use:
Customers using Gibco Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system:
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.