ESPL1 (phospho S1073) Peptide
产品名称： ESPL1 (phospho S1073) Peptide
英文名称： ESPL1 (phospho S1073) Peptide
产品编号： P1412
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ESPL1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S1073 of human ESPL1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Processing, localization, and requirement of human separase for normal anaphase progression.
Chestukhin A, Pfeffer C, Milligan S, DeCaprio JA, Pellman D.Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4574-9. Epub 2003 Apr 2.
- 2.
- Regulation of human separase by securin binding and autocleavage.
Waizenegger I, Gimenez-Abian JF, Wernic D, Peters JM.Curr Biol. 2002 Aug 20;12(16):1368-78.
- 3.
- Caspase proteolysis of the cohesin component RAD21 promotes apoptosis.
Chen F, Kamradt M, Mulcahy M, Byun Y, Xu H, McKay MJ, Cryns VL.J Biol Chem. 2002 May 10;277(19):16775-81. Epub 2002 Mar 1.
- Applications
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ESPL1 (phospho S1073) polyclonal antibody (Cat # PAB0393) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 9700
- Protein Accession#:
- NP_036423;Q14674
- Gene Name:
- ESPL1
- Gene Alias:
- ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN
- Gene Description:
- extra spindle pole bodies homolog 1 (S. cerevisiae)
- Omim ID:
- 604143
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 604359) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM
- Other Designations:
- extra spindle poles like 1,separin, separase
- Gene Pathway