服务项目

(Service Items)

周期（周）

Time(weeks)

多肽

peptide

小分子

Molecular

磷酸基/甲基/乙基化多肽

Phosphate/Methyl/acetyl-specific

多肽设计与合成

Peptide design   & synthesis

多肽设计

Peptide design

~2

可选，免费

多肽合成

Peptide   synthesis

√

√

小分子或多肽偶联

conjugation

√

√

√

免疫阶段

Immunization

免疫比格犬1只

Immunized 1 dog

~8

√

√

√

ELISA检测

ElISA detection

√

√

√

cDNA制备

cDNA preparation

逆转录制备cDNA文库

prepare cDNA   library by RT-PCR

~1

√

√

√

cDNA 文库质检

cDNA library QC   check

√

√

√

构建Fab抗体库

Fab library   construction

VH与VL基因获得

obtained VH   and VL genes

10~15

√

√

√

构建初级抗体库

primary antibody   library construction

√

√

√

构建Fab抗体库

Fab library   construction

√

√

√

噬菌体筛选

phage screening

噬菌体颗粒包装

phage particles   package

10~15

√

√

√

磁珠亲和筛选

magnetic beads   affinity screening

√

√

√

阳性克隆回收基因测序

Recover positive   clones and DNA sequencing

√

√

√

抗体表达

Antibody   expression

特异性双链基因合成与Fc基因桥接

synthetic of   target phage gene and Fc gene

~5

√

√

√

构建真核表达载体

Eukaryotic   system expression plasmids construction

√

√

√

真核表达

Eukaryotic   system expression

√

√

√

稳转细胞系构建

Stably transfected   cell line construction

纯化与检测

Purification and   detection

标签纯化

Tag affinity   purification

~1

√

√

√

特异性亲和纯化

Antigen specific   affinity purification

√

√

√

总周期（月）

Total lead   time(months)

7~9