FITC标记的E3泛素连接酶NEDD4结合蛋白1抗体
产品名称： FITC标记的E3泛素连接酶NEDD4结合蛋白1抗体
英文名称： Anti-N4BP1/FITC
产品编号： HZ-18984R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-N4BP1/FITC Conjugated antibody
FITC标记的E3泛素连接酶NEDD4结合蛋白1抗体
| 英文名称 | Anti-N4BP1/FITC |
| 中文名称 | FITC标记的E3泛素连接酶NEDD4结合蛋白1抗体 |
| 别 名 | FLJ31821; KIAA0615; MGC176730; N4BP1; N4BP1_HUMAN; NEDD4 binding protein 1; NEDD4-binding protein 1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 100kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human N4BP1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: N4BP1 is an 896 amino acid protein that belongs to the N4BP1 family. N4BP1 primarily localizes to nucleolus, however it also localizes to PML nuclear bodies when desumoylated. Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary, N4BP1 is phosphorylated upon DNA damage probably by ATM or ATR. N4BP1 interacts with Nedd4, a protein with a wide range of activities including modification of ubiquitin-protein ligase, regulation of membrane channels and potentiating hormone-dependent activation of transcription. N4BP1 inhibits the E3 ubiquitin-protein ligase ITCH, by interacting with the second WW domain of ITCH thereby competing with ITCH's substrates and impairing ubiquitination of substrates. N4BP1 has no obvious transmembrane regions, which raises the possibility that N4BP1 represents a soluble adaptor that could sequester ITCH within the cytosol. The N4BP1 gene maps to chromosome 16q12.1. Function: Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. Subcellular Location: Nucleus; nucleolus. Nucleus; PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. Tissue Specificity: Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. Post-translational modifications: Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the N4BP1 family. Database links: Entrez Gene: 9683 Human Entrez Gene: 80750 Mouse Entrez Gene: 291921 Rat SwissProt: O75113 Human SwissProt: Q6A037 Mouse Unigene: 511839 Human Unigene: 25117 Mouse Unigene: 428374 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
N4BP1是属于N4BP1家族的896个氨基酸。N4BP1主要定位于核仁，但在去甲酰化时也定位于PML核体。在心、肺、脑、肝、骨骼肌、胰腺、肾、脾、睾丸和卵巢中检测到N4BP1在DNA损伤时可能被ATM或ATR磷酸化。N4BP1与Nedd4相互作用，该蛋白具有广泛的活性，包括修饰泛素蛋白连接酶、调节膜通道和增强激素依赖的转录激活。N4BP1通过与ITCH的第二WW结构域相互作用，抑制E3泛素-蛋白连接酶ITCH，从而与ITCH的基底竞争并损害底物的泛素化。N4BP1没有明显的跨膜区，这增加了N4BP1代表一个可溶性适配子的可能性，它可以将ITCH隔离在细胞质中。N4BP1基因定位于染色体16q2.1。