CD151,CD151抗体

产品编号HZ-2524R
英文名称CD151
中文名称CD151抗体
别 名CD151 antigen; CD151 molecule; GP27; Membrane glycoprotein SFA1; MER2; PETA3; Platelet endothelial tetraspan antigen 3; RAPH; SFA 1; SFA1; Tetraspanin24; TSPAN24; CD151_HUMAN; Platelet-endothelial tetraspan antigen 3; PETA-3; Tetraspanin-24; Tspan-24; CD151.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 细胞膜受体 细胞粘附分子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Cow, Horse,
CD151,CD151抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CD151
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CD151,CD151抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008].

Function:
Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.

Subunit:
Interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, with CD9 and CD181.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.

DISEASE:
Nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]: A disorder characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

Gene ID:
977

Database links:
Entrez Gene: 977 Human
Omim: 602243 Human
SwissProt: P48509 Human
Unigene: 654379 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD151蛋白是四跨膜超家族蛋白(transmembrane-4 superfamily，TM4SF)成员，参与调节细胞的形态和极性、促进细胞黏附和迁移，并在体外血管形成中发挥着重要作用，该蛋白与血管新生有关。

产品图片

Tissue/cell: Human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CD84/SLAMF5 Polyclonal Antibody, Unconjugated(HZ-2520R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining