产品名称： 内皮细胞培养基
英文名称：Endothelial Cell Medium（ECM）
货号：1001
品牌：ScienCell
产地：美国
规格：500ml
 
 
 
Product Description 
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS,  
Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal  
growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential  
and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth  
factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is  
HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an  
atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to  
provide an optimally balanced nutritional environment that selectively supports the growth of  
normal endothelial cells in vitro. 
Components 
ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell  
Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat.  
#0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separately to  
make the complete ECM. 
Product Use 
ECM is for research use only. It is not approved for human or animal use, or for application in in  
vitro diagnostic procedures. 
Storage 
Store the basal medium at 4o C and the ECGS, FBS and P/S solution at -20o C. Protect from light.  
Shipping 
Basal medium: room temperature. Supplements: dry ice.  
Instructions for Use 
Thaw ECGS, FBS and P/S solution at 37o C. Gently tilt the tubes several times to ensure the  
contents are completely mixed before adding to the medium. Spray the medium bottle and tubes  
with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without  
touching the interior threads with fingers. Add ECGS, FBS and P/S solution to the medium and  
mix well. Since several components are light-labile, the medium should not be exposed to light  
for extended periods. We do not recommend warming medium in a 37o C water bath prior to use.  
When stored in the dark at 4o C, the reconstituted medium is stable for one month. 
Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate  
precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.