重组人VEGF165-RNAi技术-试剂-生物在线
上海沪震实业有限公司
重组人VEGF165

重组人VEGF165

商家询价

产品名称: 重组人VEGF165

英文名称: Recombinant Human Vascular Endothelial Growth Factor165

产品编号: HZ4839

产品价格: 0

产品产地: 中国/上海

品牌商标: 沪震生物

更新时间: 2023-08-17T10:24:20

使用范围: null

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 重组人VEGF165

中文名称:重组人VEGF165
英文名称:Recombinant Human Vascular Endothelial Growth Factor165
产品规格:2μg
分子量:Recombinant human VEGF165 is a 38.6kDadisulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains with Met at N-terminus.
储存条件:冷冻(-20℃)

概述

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells,which stimulates vasculogenesis and angiogenesis.VEGFs normal function is to create new blood vessels during embryonic development,new blood vessels after injury,muscle following exercise,and new vessels(collateral circulation)to bypass blocked vessels.Humans express alternately spliced isoforms of 121,145,165,183,189,and 206 amino acids(a.a.)in length.VEGF production can be induced in cells that are not receiving enough oxygen.VEGF165 appears to be the most abundant and potent isoform,followed by VEGF121 and VEGF189.Recombinant human VEGF165 contains 165 amino acids residues and it is a disulfide-linked homodimer.In addition,it shares 88%a.a.with corresponding regions of mouse and rat,96%with porcine,95%with canine,and 93%with feline,equine and bovine VEGF,respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观 Sterile Filtered White lyophilized(freeze-dried)powder.
纯度 >95%by SDS-PAGE and HPLC analyses.
来源 Escherichia coli.
生物活性 Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC)is between 1.0-8.0ng/ml.
成分 Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素 Less than 1 EU/μg of rHuVEGF165 as determined by LAL method.
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列 MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

 

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