YL369-N2 Supplement-AF

Description

YL369-Biotool™ N2 Supplement-AF is a chemically defined, serum-free and animal component-free cell culture supplement based on Bottenstein’s N1 formulation[1]. YL369-Biotool™ N2 Supplement-AF was used for the derivation, maintenance, expansion and/or differentiation of human and mouse embryonic stem (ES) cells[2-4] and tissue-derived neural stem (NS) cells [5-7]. This product is provided as a 100X concentrate and is intended for use with Neurobasal media. YL369-Biotool™ N2 Supplement-AF may be used with Neurobasal media supplemented with growth factors such as β-FGF and EGF or used with DMEM. YL369-Biotool™ N2 Supplement-AF is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

Product Specifications

Size	5 mL
Form	Liquid
Concentration	100X
Classification	Serum-Free, Animal component-free
Cell Type	Neuronal Tumor Cells, Neuronal Cells, Stem Cells
Purity or Quality Grade	Cell Culture Grade
Shipping	Dry Ice

Product Formulation

Components	Molecular Weight	Concentration (mg/L)	mM
Proteins
Human Transferrin (Holo)	10000.0	10000.0	1.0
Insulin (Recombinant Full Chain)	5807.7	500.0	0.0860926
Other Components
Progesterone	314.47	0.63	0.0020033708
Putrescine	161.0	1611.0	10.006211
Selenite	173.0	0.52	0.0030057803

Storage & stability

Upon receipt, store at -20°C in the dark (stable for 6 months) until ready to use. Once combined with media, store at 4°C in the dark and use within 4 weeks.

Caution

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures.

更多详情请访问唯一官方网站http://www.biotool.com/product/yl369-n2-supplement-af.html