分子量:47.1 kDa

纯度:>90% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Carboxypeptidase M, His Tag by the LAL method.

Buffer:50 mM MES, pH 6.5 with 100 mM NaCl

生物活性:Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde. The specific activity is >40,000 pmoles/min/μg.

产品特性:Human Carboxypeptidase M, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The predicted N-terminus is Leu 18. The reducing (R) protein migrates as 49 kDa in SDS-PAGE  due to glycosylation.

产品背景:Carboxypeptidase M (CPM) belongs to the peptidase M14 family. CPM specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins, and the activity is inhibited by O-phenanthroline and MGTA and activated by cobalt. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.