预优化siRNA转染试剂-MEFs
产品名称： 预优化siRNA转染试剂-MEFs
英文名称： GenMute™ siRNA Transfection Reagent for MEFs
产品编号： SL100568-MEF
产品价格： 0
产品产地： 美国
品牌商标： SignaGen
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 济南市经六路689号绿地·玫瑰坊A座734号
- 邮编 : 250021
- 所在区域 : 山东
- 电话 : 159****9896 点击查看
- 传真 : 点击查看
- 邮箱 : jnqsw@163.com; info@signagen-china.com
Description:
GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Refer to the following optimal transfection conditions for maximal silencing on MEFs. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
|
Summary of Optimal Transfection Conditions: |
|
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol .pdf