FITC标记的染色体特异性转录延伸因子抗体
产品名称： FITC标记的染色体特异性转录延伸因子抗体
英文名称： Anti-SUPT16H/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-SUPT16H/FITC Conjugated antibody
FITC标记的染色体特异性转录延伸因子抗体
| 英文名称 | Anti-SUPT16H/FITC |
| 中文名称 | FITC标记的染色体特异性转录延伸因子抗体 |
| 别 名 | CDC68; Chromatin specific transcription elongation factor 140 kDa subunit; Chromatin-specific transcription elongation factor 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; FACT 140 kDa subunit; FACT; FACT complex subunit SPT16; FACTP140; FLJ10857; FLJ14010; hSPT16; SP16H_HUMAN; Suppressor of Ty 16 homolog; Supt16h. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 转录调节因子 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 120kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SUPT16H/CDC68 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Function: Component of the FACT complex, a general chromatinfactor that acts to reorganize nucleosomes. The FACT complex isinvolved in multiple processes that require DNA as a template suchas mRNA elongation, DNA replication and DNA repair. Duringtranscription elongation the FACT complex acts as a histonechaperone that both destabilizes and restores nucleosomalstructure. It facilitates the passage of RNA polymerase II andtranscription by promoting the dissociation of one histone H2A-H2Bdimer from the nucleosome, then subsequently promotes thereestablishment of the nucleosome following the passage of RNApolymerase II. The FACT complex is probably also involved inphosphorylation of 'Ser-392' of p53/TP53 via its association withCK2 (casein kinase II). Also involved in vitamin D-coupledtranscription regulation via its association with the WINACcomplex, a chromatin-remodeling complex recruited by vitamin Dreceptor (VDR), which is required for the ligand-bound VDR-mediatedtransrepression of the CYP27B1 gene (By similarity). Subunit: Component of the FACT complex, a stable heterodimer ofSSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complexwhich forms following UV irradiation, composed of SSRP1, SUPT16H,CSNK2A1, CSNK2A2 and CSNK2B. Component of the WINAC complex, atleast composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2,SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A andTOP2B. Interacts with NEK9. Binds to histone H2A-H2B. Interactswith GTF2E2. Subcellular Location: Nucleus. Chromosome. Note=Colocalizes withRNA polymerase II on chromatin. Recruited to actively transcribedloci. Tissue Specificity: Ubiquitous. Post-translational modifications: ADP-ribosylated. ADP-ribosylation by PARP1 is induced bygenotoxic stress and correlates with dissociation of FACT fromchromatin. Similarity: Belongs to the peptidase M24 family. SPT16 subfamily. Database links: Entrez Gene: 514989 Cow Entrez Gene: 612874 Dog Entrez Gene: 11198 Human Entrez Gene: 114741 Mouse Entrez Gene: 305851 Rat Omim: 605012 Human SwissProt: E1BNP8 Cow SwissProt: Q9Y5B9 Human SwissProt: Q920B9 Mouse Unigene: 213724 Human Unigene: 21662 Mouse Unigene: 93187 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
事实复合物的组成部分，是一种重组核小体的一般染色质因子。事实上复杂的涉及多个过程，需要DNA作为模板，如mRNA的伸长，DNA复制和DNA修复。在转录延伸过程中，事实复合物作为组蛋白伴侣，既破坏了稳定，又恢复了核小体结构。它促进RNA聚合酶II和转录的通过促进一个组蛋白H2A- H2B二聚体从核小体的解离，然后在RNA聚合酶II的通路之后促进核小体的重建。事实上复杂的可能也涉及磷酸化的“Ser-39 2”的p53 / TP53通过其与CK2（酪蛋白激酶II）的关联。还涉及维生素D偶联转录调节通过其与WiAC复合物，由维生素D受体（VDR）招募的染色质重塑复合物，这是配体结合的VDR介导的CYP27 B1基因的阻遏所必需的。