AIF抗体
Applications
| Tested Applications: |
ELISA, IHC, WB, IP, IF |
| Cited applications |
WB |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Human |
| Positive WB detected in |
Jurkat cells, A549 cells, HeLa cells, rat kidney tissue |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human kidney tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:1000-1:10000
IP : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:10-1:100 |
AIF抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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Jurkat cells were subjected to SDS PAGE followed by western blot with 17984-1-AP(AIF antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human kidney using 17984-1-AP(AIF antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using 17984-1-AP(AIF antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-AIF (IP:17984-1-AP, 3ug; Detection:17984-1-AP 1:2000) with HeLa cells lysate 1320ug.
Immunofluorescent analysis of HepG2 cells using 17984-1-AP( AIF Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product Information
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information AIF抗体
| Immunogen: |
AIF fusion protein ag12400 |
Full Name: |
Apoptosis-inducing factor, mitochondrion-associated, 1 |
Caculated Molecular
Weight: |
609aa,66 kDa |
Observed Molecular
Weight: |
67kd |
| GenbankNo |
BC111065 |
GeneID: |
9131 |
| Symbol |
AIFM1 |
Synonyms |
AIF, AIFM1, PDCD8 |
Background
|
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058). AIF抗体
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