FITC标记的磷酸化相关死亡促进因子抗体
产品名称： FITC标记的磷酸化相关死亡促进因子抗体
英文名称： Anti-phospho-BAD(Ser91)/FITC
产品编号： HZ-12567R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-BAD(Ser91)/FITC Conjugated antibody
FITC标记的磷酸化相关死亡促进因子抗体
| 英文名称 | Anti-phospho-BAD(Ser91)/FITC |
| 中文名称 | FITC标记的磷酸化相关死亡促进因子抗体 |
| 别 名 | Bad (phospho S91); p-Bad (phospho S91); BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-like protein 8; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; BclXL; Proapoptotic BH3 Only Protein. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 细胞凋亡 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human BAD around the phosphorylation site of Ser91 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform. This antibody recognise the phosphorylation site of human Ser75, mouse Ser111, rat Ser112. Function: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. Subcellular Location: Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. Tissue Specificity: Expressed in a wide variety of tissues. Post-translational modifications: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. Similarity: Belongs to the Bcl-2 family. Database links: Entrez Gene: 572 Human Omim: 603167 Human SwissProt: Q92934 Human Unigene: 370254 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
Bad是Bcl2家族的成员，通过与存活蛋白Bcl2和BclxL形成异二聚体来促进细胞凋亡，从而阻止它们与BAX结合。Bad存在于外线粒体膜上，一旦在生长刺激下被磷酸化，就会转移到细胞质中。不良状态的磷酸化状态是死亡或细胞存活的关键检查点。JNK诱导的BAD丝氨酸128磷酸化通过拮抗生长因子对Bad介导的细胞凋亡的抑制作用而促进Bad的凋亡作用。CDC2诱导的丝氨酸128的磷酸化对其与14-3-3蛋白的相互作用具有抑制作用。后者的相互作用对于丝氨酸155上的Bad磷酸化至关重要，该位点位于BH3结构域内，导致BclxL的释放和促进细胞存活。该基因的选择性剪接导致两个转录变体，编码相同的异构体。该抗体识别人Ser75、小鼠Ser111、大鼠Ser112的磷酸化位点。