TRIM7,糖原生成素相互作用蛋白抗体

产品编号HZ-9164R
英文名称TRIM7
中文名称糖原生成素相互作用蛋白抗体
别 名Glycogenin-interacting protein; GNIP; RING finger protein 90; RNF90; TRIM7; TRIM7_HUMAN; Tripartite Motif Containing 7; Tripartite motif protein TRIM 7; Tripartite motif-containing protein 7.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Sheep,
TRIM7,糖原生成素相互作用蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TRIM7/RNF90
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TRIM7,糖原生成素相互作用蛋白抗体PubMedPubMed
产品介绍background:
The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM7 (tripartite motif-containing 7), also known as RNF90 or GNIP, is a 511 amino acid protein that belongs to the TRIM family and contains one RING-type zinc finger, one B box-type zinc finger and one SPRY domain. Expressed in placenta and skeletal muscle and present at lower levels in brain, heart and pancreas, TRIM7 localizes to both the cytoplasm and the nucleus where it exists as dimers and is thought to participate in the initiation of glycogen synthesis. Multiple isoforms of TRIM7 exist due to alternative splicing events.

Subunit:
Forms homodimers and heterodimers. Interacts with GYG and DES.

Tissue Specificity:
Skeletal muscle and placenta, at lower levels in heart, brain and pancreas. Isoform 1 is widely expressed with high level in testis, kidney and heart.

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

Gene ID:
81786

TRIM7,糖原生成素相互作用蛋白抗体Database links:
Entrez Gene: 81786 Human
Omim: 609315 Human
SwissProt: Q9C029 Human
Unigene: 487412 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Lane 1: (human/cell line)Sihalysatesprobed withTRIM7Polyclonal Antibody, Unconjugated (Catalog #HZ-9164R) at 1:300 overnight at 4ⅹC. Followed by a conjugated secondary antibody (Secondary Catalog #926-32211) at 1:10000 for 60 min at 37ⅹC. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM7) Polyclonal Antibody, Unconjugated (HZ-9164R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.