FITC标记的磷酸化G蛋白偶联受体激酶相互作用蛋白1
产品名称： FITC标记的磷酸化G蛋白偶联受体激酶相互作用蛋白1
英文名称： Anti-phospho-GIT1(Tyr545) /FITC
产品编号： HZ-5373R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-phospho-GIT1(Tyr545) /FITC Conjugated antibody
FITC标记的磷酸化G蛋白偶联受体激酶相互作用蛋白1
| 英文名称 | Anti-phospho-GIT1(Tyr545) /FITC |
| 中文名称 | FITC标记的磷酸化G蛋白偶联受体激酶相互作用蛋白1 |
| 别 名 | GIT1 (phospho Y545); p-GIT1 (phospho-Y545); ARF GAP GIT1; ARF GTPase activating protein GIT1; CAT 1; Cat1; Cool associated and tyrosine phosphorylated protein 1; G protein coupled receptor kinase interactor 1; GRK interacting protein 1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 84kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr545 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2). G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4). GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6). GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5). Function: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Subcellular Location: Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin. Post-translational modifications: Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase. Similarity: Contains 3 ANK repeats. Contains 1 Arf-GAP domain. Database links: Entrez Gene: 28964 Human Entrez Gene: 216963 Mouse Entrez Gene: 83709 Rat Omim: 608434 Human SwissProt: Q9Y2X7 Human SwissProt: Q68FF6 Mouse SwissProt: Q9Z272 Rat Unigene: 514051 Human Unigene: 290182 Mouse Unigene: 34521 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
异源三聚体G蛋白介导的信号转导是一个动态调节的过程，信号强度随着时间的推移而减少，尽管激动剂（1，2）持续存在。G蛋白偶联受体激酶（GRKs）是由活化的G蛋白偶联受体激活的，它们的功能是在异三聚体G蛋白信号级联（3,4）中磷酸化和失活细胞表面受体。GIT1（GRK相互作用因子1）和GIT2是GTP酶激活蛋白（GAP），其成员为小GTP结合蛋白ADP核糖基化因子（ARF）家族，参与囊泡运输（5，6）。GIT1过表达导致B2肾上腺素受体的内化和再敏感减少，从而降低B2肾上腺素能受体信号（5）。