FITC标记的半胱胺酸蛋白酶-10抗体
产品名称： FITC标记的半胱胺酸蛋白酶-10抗体
英文名称： Anti-Caspase-10/FITC
产品编号： HZ-0092R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-Caspase-10/FITC Conjugated antibody
FITC标记的半胱胺酸蛋白酶-10抗体
| 英文名称 | Anti-Caspase-10/FITC |
| 中文名称 | FITC标记的半胱胺酸蛋白酶-10抗体 |
| 别 名 | ALPS 2; ALPS2; Apoptosis related cysteine peptidase; Apoptotic protease Mch 4; CASP 10; CASP10; CASP10 protein; Caspase 10 apoptosis related cysteine peptidase; Caspase 10 apoptosis related cysteine protease; Caspase10; FADD Like Ice 2; Fas Associated Death Domain Protein; FAS associated death domain protein interleukin 1B converting enzyme 2; FLICE 2; FLICE2; ICE Like Apoptotic Protease 4; Interleukin 1B Converting Enzyme 2; MCH 4; MCH4. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 免疫学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 59kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caspase-10 subunit p12 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Caspases are a family of intracellular proteases that mediate cell death and are the principal effectors of apoptosis. Caspase 10 (Mch4, ICE-LAP4, FLICE2) plays an important role in apoptosis induced by a variety of inducers such as TNF alpha and Anti-Fas antibody. It is a large prodomain caspase classified together with caspases 2, 8, and 9 as a signaling caspase. Four isoforms of caspase 10 (caspase 10a, 10b, 10c, and 10d) having the same prodomain but different mature large and small subdomain, have been described. Caspase 10 contains two death domains (DED) involved in linking to the death effector domain of the adapter protein FADD and recruiting the complex to TNFR1 and Fas. The inactive procaspase 10 is variably expressed in many tissues and cell lines as a cytosolic protein. The mature form of caspase 10 comprises two subunits, p23/p17 (splice isoforms) and p12. Interestingly, a caspase 9- dependent processing of caspase 10 by caspase 6 in cell-free extracts has recently been suggested. Caspase 10 can cleave and activate caspases 3, 4, 6, 7, 8, and 9. This is followed by cleavage of numerous key proteins, including the nuclear protein PARP. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Tissue Specificity: Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Post-translational modifications: Cleavage by granzyme B and autocatalytic activity generate the two active subunits. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects. Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. Database links: Entrez Gene: 843 Human Omim: 601762 Human SwissProt: Q92851 Human Unigene: 5353 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
半胱氨酸天冬氨酸蛋白酶是一类介导细胞死亡的细胞内蛋白酶家族，是细胞凋亡的主要作用因子。Caspase 10(Mch4、ICE-LAP4、FLICE2)在TNF-α、抗Fas抗体等多种诱导剂诱导的细胞凋亡中起重要作用。这是一个大的前域caspase分类与半胱氨酸天冬氨酸蛋白酶2, 8，和9作为信号半胱天冬酶。本文报道了四种caspase 10亚型(caspase 10a、10b、10c和10d)，它们具有相同的前域，但不同的成熟大小亚域。Caspase 10包含两个死亡结构域（DED），涉及连接至适配蛋白FADD的死亡效应器结构域和将复合物募集到TNFR1和Fas。不活跃的脯氨酶10作为胞浆蛋白在许多组织和细胞系中可变地表达。caspase 10的成熟形式包括两个亚基，p23／p17（剪接异构体）和p12。有趣的是，最近提出了caspase 9依赖半胱氨酸蛋白酶10的caspase 6在无细胞提取物中的处理。半胱氨酸天冬氨酸蛋白酶10可以裂解并激活胱天蛋白酶3, 4, 6、7, 8和9。其次是许多关键蛋白的裂解，包括核蛋白PARP。