EPHA3 Validated Chimera RNAi
产品名称： EPHA3 Validated Chimera RNAi
英文名称： EPHA3 Validated Chimera RNAi
产品编号： H00002042-R01V
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1, mRNA.
- Reactivity:
- Human
- Storage Instruction:
- Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.
- Supplied Product:
- DEPC water
- Target Refseq:
- NM_005233
- Note:
- Position of the Chimera RNAi.
-
- Publication Reference
- 1.
- dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
- 2.
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11.
- 3.
- Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.
- 4.
- siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.
- Applications
- RNAi Knockdown (Antibody validated)
- Western blot analysis of EPHA3 over-expressed 293 cell line, cotransfected with EPHA3 Validated Chimera RNAi ( Cat # H00002042-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EPHA3 monoclonal antibody (M01) clone 3A12 (Cat # H00002042-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 2042
- Gene Name:
- EPHA3
- Gene Alias:
- ETK,ETK1,HEK,HEK4,TYRO4
- Gene Description:
- EPH receptor A3
- Omim ID:
- 179611
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq
- Other Designations:
- TYRO4 protein tyrosine kinase,eph-like tyrosine kinase 1,ephrin receptor EphA3,human embryo kinase 1
- Gene Pathway
- Related Disease