目录号 
                        
规格 
                        
价格 
                        
说明书下载 
                    
                    

                        
C604A
                        
2μg
                        
400元
                        

                    
                    

                        
C604B
                        
10μg
                        
1800元
                    
                    

                        
C604C
                        
50μg
                        
4500元
                    
                
            
            
        
        

            

            
 
            
Endothelin-3, also known as EDN3, EDN-3, ET-3,Preproendothelin-3, PPET3, WS4B and HSCR4, is a 21 amino acid peptide processed from the precursor protein. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. EDN3 interacts with endothelin receptor B, on the surface of cells. Throughout embryonic development, EDN3 takes part in neural crest cells that migrate from the developing spinal cord to specific regions in the embryo, where they give rise to many different types of cells. EDN3 and EDN3R are necessary for the formation of nerves in the large intestine (enteric nerves) and melanocytes (produce melanin). Defects in EDN3 are the cause of Hirschsprung disease type 4 (HSCR4), also known as aganglionic megacolon (MGC) and the cause of congenital central hypoventilation syndrome (CCHS) which also known as congenital failure of autonomic control or Ondine curse.
            


            ·Description

            Novoprotein Recombinant Human Endothelin-3 produced by mammalian cells expression system.Target protein is expressed with sequence (Cys97-Trp117) of Human endothelin-3 (Uniprot #P14138) fused with a polyhistidine tag at the C-terminus.
            


            ·Quality Control
            
Purity: Greater than 90.0% as determined by: 

            (a) Analysis by RP-HPLC. 

            (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 

             
            
Endotoxin:Less than 0.1ng/μg (1EU/μg) of Endothelin-3 (Human)
            
 
            
·Formulation

              Novoprotein Recombinant Human EDN3 was lyophilized at 1mg/ml in 20mM PBS.
            
·Reconstitution

            We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human EDN3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.
            
·Storage 

            Lyophilized Recombinant Human EDN3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human EDN3 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.
            
 
            
Please avoid freeze-thaw cycles.