TSPAN6,四分子交联体6抗体

产品编号HZ-9415R
英文名称TSPAN6
中文名称四分子交联体6抗体（四旋蛋白）
别 名Putative NF kappa B activating protein 321; Putative NF-kappa-B-activating protein 321; T245; T245 protein; Tetraspan TM4SF; Tetraspanin 6; Tetraspanin TM4 D; Tetraspanin TM4-D; Tetraspanin TM4D; Tetraspanin-6; Tm4sf; TM4SF6; Transmembrane 4 superfamily member 6; TSN6_HUMAN; TSPAN 6; Tspan-6; TSPAN6; Tspan6 protein.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 染色质和核信号 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Horse, Rabbit, Sheep,
TSPAN6,四分子交联体6抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TSPAN6
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TSPAN6,四分子交联体6抗体PubMedPubMed
产品介绍background:
Tetraspanins are a group of hydrophobic membrane proteins that interact with a wide variety of proteins including intracellular signaling molecules, integrins and membrane receptors. Members of the tetraspanin family are characterized by the presence of four hydrophobic domains and play a role in cell development, activation, growth and motility. TSPAN6 (tetraspanin-6), also known as A15 homolog, putative NF-kappa-B-activating protein 321, T245 protein, tetraspanin TM4-D or TM4SF6 (transmembrane 4 superfamily member 6), is a 245 amino acid multi-pass membrane protein that belongs to the tetraspanin (TM4SF) family. TSPAN6 is expressed at high levels in brain, with lower levels of expression found in colon, lung, pancreas, prostate, retina and melanocytes, as well as fetal heart, liver and spleen. The gene encoding TSPAN6 maps to the human X chromosome.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

Gene ID:
7105

Database links:
Entrez Gene: 7105 Human
Omim: 300191 Human
SwissProt: Q32KU6 Cow
SwissProt: O43657 Human
SwissProt: Q5RAS5 Orangutan
Unigene: 43233 Human

TSPAN6,四分子交联体6抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TSPAN6 Polyclonal Antibody, Unconjugated(HZ-9415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TSPAN6 Polyclonal Antibody, Unconjugated(HZ-9415R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TSPAN6 Polyclonal Antibody, Unconjugated(HZ-9415R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining