PGK1 polyclonal antibody
产品名称： PGK1 polyclonal antibody
英文名称： PGK1 polyclonal antibody
产品编号： PAB2036
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PGK1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human PGK1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases.
Daly EB, Wind T, Jiang XM, Sun L, Hogg PJ.Biochim Biophys Acta. 2004 Apr 1;1691(1):17-22.
- 2.
- Regulation of urokinase receptor expression by phosphoglycerate kinase.
Shetty S, Muniyappa H, Halady PK, Idell S.Am J Respir Cell Mol Biol. 2004 Jul;31(1):100-6. Epub 2004 Feb 5.
- 3.
- Blood level of phosphoglycerate kinase does not correlate with presence or extent of tumor.
Daly EB, Chen G, Sun L, Friedlander M, Goldstein D, Beer DG, Chesterman CN, Hogg PJ.Int J Biol Markers. 2004 Apr-Jun;19(2):170-2.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of PGK1 polyclonal antibody (Cat # PAB2036) in HeLa cell line lysate (35 ug/lane). PGK1 (arrow) was detected using the purified Polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with PGK1 polyclonal antibody (Cat # PAB2036) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5230
- Protein Accession#:
- NP_000282;Q5J7W1
- Gene Name:
- PGK1
- Gene Alias:
- MGC117307,MGC142128,MGC8947,MIG10,PGKA
- Gene Description:
- phosphoglycerate kinase 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq
- Other Designations:
- OTTHUMP00000023595,cell migration-inducing gene 10 protein,primer recognition protein 2
- Gene Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
- Metabolic pathways
- Related Disease