高尔基蛋白73(GP73)重组蛋白

YB668Mu021
Recombinant Golgi Protein 73 (GP73)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 高尔基蛋白73(GP73)重组蛋白[ PROPERTIES ]
Residues: Val119~Gly223 (Accession # Q8NBJ4),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Golgi apparatus, cis-Golgi
network membrane; Single-pass type II
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
 高尔基蛋白73(GP73)重组蛋白0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 44.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
 高尔基蛋白73(GP73)重组蛋白DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-VL QDQLKTLQRN
YGRLQQDVLQ FQKNQTNLER KFSYDLSQCI NQMKEVKEQC EERIEEVTKK GNEAVASRDL
SENNDQRQQL QALSEPQPRL QAAGLPHTEV PQG
[ REFERENCES ]
1. Kladney R.D., et al. (2000) Gene 249:53-65. 2. Kladney R.D., et al. (2002) Hepatology 35:1431-1440. 3. Kladney R.D., et al. (2002) Virology 301:236-246. 4. Denais C., et al. (2011) Hum. Mutat. 32:231-239.